Basic Research on Fibroblast Migration / Papers of The 8th Japan Scar Workshop


Papers of The 8th Japan Scar Workshop
3. Basic Research on Fibroblast Migration Shigeyuki Kanazawa, Tateki Kubo, Ko Hosokawa Department of Plastic Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
Fibroblast proliferation and migration play important roles in wound healing. bFGF is known to promote both fibroblast proliferation and migration during the wound healing process. However, signal transduction of bFGF-induced fibroblast migration is still unclear, as bFGF can affect both proliferation and migration. Herein, we investigated the effect of bFGF on fibroblast migration regardless of its effect on fibroblast proliferation. We noticed involvement of the small GTPases of the Rho family, PI3-kinase, and JNK. bFGF activated RhoA, Rac1, PI3-kinase, and JNK in cultured fibroblasts. Inhibition of RhoA did not block bFGF-induced fibroblast migration, whereas inhibition of Rac1, PI3-kinase, or JNK blocked fibroblast migration significantly. PI3-kinase-inhibited cells down-regulated the activities of Rac1 and JNK, and Rac1-inhibited cells down-regulated JNK activity, suggesting that PI3-kinase is upstream of Rac1 and that JNK is downstream of Rac1. Thus, we concluded that PI3-kinase, Rac1, and JNK are essential for bFGF-induced fibroblast migration, which is a novel pathway of bFGF-induced cell migration.
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