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Papers of The 1st Japan Scar Workshop |
7. Extracellular matrix gene expression altered by N-methylethanolamine
(MEA) in human dermal fibroblasts
Yamanaka M, Ishikawa O
Department of Dermatology, Gunma University Graduate School of Medicine,
Gunma, Japan
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Tissue fibrosis develops when dysregulation of extracellular matrix (ECM)
turnover favors deposition of ECM proteins over degradation. Fibrosis
may then lead to organ dysfunction as observed in keloids and
hypertrophic scars. In the present study, we investigated the
antifibrotic properties of N-methylethanolamine (MEA). MEA suppressed
the expression of COL2(I) mRNA and its protein levels in a dose- and
time-dependent manner in human dermal fibroblasts. Under the same
conditions, MEA enhanced the expression of matrix metalloproteinase-1
(MMP-1) mRNA and its protein levels. Together, these observations
suggest that MEA may inhibit ECM deposition . We investigated which
pathway is involved in the action of MEA. We focused on the MEK/ERK
signaling pathways. Assays using specific inhibitors of MEK/ERK
pathways, PD98059 and U0126, indicated that the MEK/ERK pathways are
involved in MEA-induced MMP1 stimulation and COL2(I) inhibition. We
confirmed that MEA induced ERK1/2 phosphorylation. In conclusion, our
study demonstrates that MEA displays an antifibrotic action by reducing
type-I collagen production and inducing MMP-1 production. Therefore, MEA
can be a promising candidate for the treatment of diseases characterized
by excessive ECM deposition such as keloids and hypertrophic scars. |
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